- 雞胚成纖維細(xì)胞
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chicken embryo fibroblast中文什么意思
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- Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
66弱毒株在雞胚成纖維細(xì)胞上的培養(yǎng) - Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection
亞群禽白血病病毒的雞胚成纖維細(xì)胞系建立 - Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk質(zhì)粒同時(shí)經(jīng)hind 、 kpn酶切后連接得中間質(zhì)粒skha ；將質(zhì)粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點(diǎn)，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質(zhì)粒puc19f用hind 、 sal酶切經(jīng)klenow酶補(bǔ)平插入到經(jīng)sma酶切后的skifn中pe / l啟動(dòng)子下獲得中間質(zhì)粒skf ，再將質(zhì)粒skf和puc18質(zhì)粒先分別用ecor 、 xho酶切klenow補(bǔ)平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點(diǎn)，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達(dá)載體pfgs11haf 。 - Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細(xì)胞系sfg上高度表達(dá)的meq蛋白產(chǎn)物免疫balb / c小鼠，然后收獲其免疫脾細(xì)胞并與腫瘤細(xì)胞系spz / 0通過peg于體外融合;獲得的雜交瘤細(xì)胞被克隆并通過與mdv感染的雞胚成纖維細(xì)胞( cef )做免疫熒光試驗(yàn)( fa ) ，進(jìn)行其分泌抗meq單克隆抗體( mcab )能力的篩選。 - From dead chicken which infected infectious stunting syndrom of our province , one virue was isolated using spfeggs , chicken embryo fibroblast , mdck18 , and vero cell . this virus was unable to agglutinate chicken erythrocytes . in order to definite the pathogeny of infectious stunting syndrom . physical and chemical specific property , types of the nucleic acid of the isolated virus , recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken
為確定該病的病原，對(duì)所分離病毒進(jìn)行了理化特性測(cè)定、病毒核酸型別測(cè)定、動(dòng)物回歸試驗(yàn)等生物學(xué)特性測(cè)定，證明該分離病毒與細(xì)小病毒科( parvovirdae )細(xì)小病毒屬( parvovirus )的雞細(xì)小病毒( chickenparvovirus )特性基本相符，核酸型為dna型。